Part:BBa_K3052010:Design

Precursor Circuit -- a series of enzymes used for GPP production

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1557
  Illegal XbaI site found at 8869
  Illegal SpeI site found at 10020
  Illegal PstI site found at 4253
  Illegal PstI site found at 6546
  Illegal PstI site found at 6837
  Illegal PstI site found at 7196
  Illegal PstI site found at 11005
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1557
  Illegal NheI site found at 3467
  Illegal SpeI site found at 10020
  Illegal PstI site found at 4253
  Illegal PstI site found at 6546
  Illegal PstI site found at 6837
  Illegal PstI site found at 7196
  Illegal PstI site found at 11005
  Illegal NotI site found at 7688
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1557
  Illegal BglII site found at 1566
  Illegal XhoI site found at 11386
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1557
  Illegal XbaI site found at 8869
  Illegal SpeI site found at 10020
  Illegal PstI site found at 4253
  Illegal PstI site found at 6546
  Illegal PstI site found at 6837
  Illegal PstI site found at 7196
  Illegal PstI site found at 11005
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1557
  Illegal XbaI site found at 8869
  Illegal SpeI site found at 10020
  Illegal PstI site found at 4253
  Illegal PstI site found at 6546
  Illegal PstI site found at 6837
  Illegal PstI site found at 7196
  Illegal PstI site found at 11005
  Illegal NgoMIV site found at 1443
  Illegal NgoMIV site found at 5780
  Illegal NgoMIV site found at 7726
  Illegal AgeI site found at 8179
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The product of this circuit -- GPP is an unstable substance which can hardly be detected, so validation and parameters measurements need further effort and sophisticated experimental methods.

Source

This circuit comes from modification of a previous plasmid producing LS in literature.